Figure 1

Catalase activity and RpoS expression in representative Suc⁺⁺ mutants of VTEC strains EDL933, CL106 and EC3-377. (A) Samples were separated by native PAGE and stained for catalase activity. Catalase HPI (KatG) and HPII (KatE) are indicated. (B) Expression of RpoS and RpoS-regulated AppA by Western analysis. Mutations in rpoS were identified in these tested Suc⁺⁺ mutants by sequencing, and sequences are provided in Supplemental material Figure S1 and Figure S2. To confirm equal protein loading, identical gels were run in parallel and stained by Coomassie Blue R-250 [14, 71].