Figure 1
From: A plant MinD homologue rescues Escherichia coli HL1 mutant (ΔMinDE) in the absence of MinE
The phenotype of E. coli cells. (A) Wildtype, DH5α. (B) HL1 mutant (ΔMinDE). (C) HL1 mutant (ΔMinDE) complemented by pM1113-MinDE at 20 μM IPTG. (D) HL1 mutant (ΔMinDE) cannot be complemented by pM1113-AtMinD at 0 μM IPTG. (E) HL1 mutant (ΔMinDE) complemented by pM1113-AtMinD at 50 μM IPTG. (F) HL1 mutant (ΔMinDE) containing pM1113-MinD at 20 μM IPTG. (G) RC1 mutant (ΔMinCDE). (H) RC1 mutant (ΔMinCDE) containing pM1113-AtMinD at 50 μM IPTG. Arrows in (B, D, G and H) mark the minicells. The bar in (A to E, G and H) represents 10 μm; the bar in (F) represents 20 μm.