Table 2 Plasmids used in this study

Cloning vectors

pGemTEasy

T/A cloning

Promega

pMV306H

mycobacterial integrating vector, Hyg^R

Med-Immune Inc.

pMV306K

mycobacterial integrating vector, Kan^R

Med-Immune Inc.

pMV261

mycobacterial Escherichia coli shuttle vector, carrying heat shock (P_{hsp 65}) promoter, Km^R

Med-Immune Inc.

RpoB expression vectors

pMRP1

wild type rpoB of M. tuberculosis H₃₇Ra controlled by natural promoter P _rpoB cloned in integration vector pMV306K, Kan^R

This study

pMRP2-9

mutated rpoB of M. tuberculosis clinical strains controlled by natural promoter P _rpoB cloned in integration vector pMV306K; 2-represents H526D; 3-D516V; 4-Q510H/D516Y; 5-S512I/D516G; 6-Q513L; 7-M515I/D516Y; 8-D516Y; 9-S531L, respectively, Kan^R

This study

pMERP1

wild type rpoB of M. tuberculosis H₃₇Ra controlled by heat shock promoter P_{hsp 65}in pMV261, Kan^R

This study

pMERP2-9

mutated rpoB of M. tuberculosis clinical strains controlled by heat shock promoter P_{hsp 65}in pMV261, 2-represents H526D; 3-D516V; 4-Q510H/D516Y; 5-S512I/D516G; 6-Q513L; 7-M515I/D516Y; 8-D516Y; 9-S531L, respectively, Kan^R

This study

pMHRP1

wild type rpoB of M. tuberculosis H₃₇Ra controlled by heat shock promoter P_{hsp 65}in pMV306, Hyg^R

This study

pMHRP2-9

mutated rpoB of M. tuberculosis clinical strains controlled by heat shock promoter P_{hsp 65}in pMV306, '2-represents H526D; 3-D516V; 4-Q510H/D516Y; 5-S512I/D516G; 6-Q513L; 7-M515I/D516Y; 8-D516Y; 9-S531L, respectively, Hyg^R

This study