Table 1 Primer sequences used for PCR amplification
Amplified region | Primer | Sequence | Product size (bp) |
|---|---|---|---|
promoter region (950 bp) and 5' part of rpoB gene (721 bp) | P-rpo-s | 5'-tctagacgagagcggcggtgcaatc | 1671 |
| Â | P-rpo-r | 5'-gctcgctggtccagcccagc | Â |
3' part of rpoB gene (1258 bp) and downstream region (218 bp) | 3'rpo-s | 5'-cgacaccaagctgggtgcgg | 1476 |
| Â | 3'rpo-r | 5'-aagcttccagtcgcgagtcggcccg | Â |
Bst EII fragment of rpoB gene including 81-bp hot spot region | bst-s | 5'-cgcgacaccgtcggcgtgcg | 1852 |
| Â | bst-r | 5'-aagtgtcgcgcacctcgcgggc | Â |
pMV306 (221 bp) and insert DNA cloned in MCS of this vector | MV-r | 5'-aaggcccagtctttcgactgagc | 221 + insert |
| Â | MV-s | 5'-gtggataaccgtattaccgcc | DNA |