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Table 3 Sequences of forward (F) and reverse (R) primers used in semi-quantitative RT-PCR to validate Xoo and Xoc gene expression changes determined by microarray analysis.

From: A two-genome microarray for the rice pathogens Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola and its use in the discovery of a difference in their regulation of hrp genes

GeneIDa Primer Sequence
XOO4289 F 5' ACA TCG CCG ATA ATT TCC AG 3'
R 5' CGC AAC ACC TTG TAC TCG AC 3'
XOO4035 F 5' GGT CTT CGG ATC GTC AAC AT 3'
R 5' GAT CAG AAA GCC GAT CTT GC 3'
XOO1994 F 5' GTT GGA GCA CAC CAT GAA AG 3'
R 5' GGT ACA GCT CCA GAC CGA TG 3'
XOO2803 F 5' CTG TTC CAA GCA GAC CCT GT 3'
R 5' CAC GAT GGG AAA CCT GAA AC 3'
XOO0424 F 5' CGG CTG AAG AAC TAC GCT TC 3'
R 5' CTT GGT CAG CTC GTT GAT GA 3'
XOO0423 F 5' CGA AGA AGG CCT CTA CAT GG 3'
R 5' CGA AGA AGG CCT CTA CAT GG 3'
XOO0076 F 5' GTG CCA CGT TGA AGT CAA GA 3'
R 5' CTC ACT TAA TTC GCG CTT CC 3'
XOO1379 F 5' GCG ATA CCA GTC CAG GAT GT 3'
R 5' CTT TTC CTC GTT GCA CTG GT 3'
XOO0094 F 5' CAC CTA CGG CTT TGT CTG GT 3'
R 5' CAT TGC CAA ATG TGT TGG AG 3'
XOO0770 F 5' ATC GGC AGG TCG TAC TTG AT 3'
R 5' GTC AGA CCC TGC TGT TCT CC 3'
XOO0282 F 5' CTG ATG AAT GAG CCT CAC GA 3'
R 5' GAT TCC ATG TAG CCC AGC AT 3'
XOO2163 F 5' AAC GGT AGA ACT TGC CAT CG 3'
R 5' AAC CTG GAC ATC CTG GAC AT 3'
XOO2757 F 5' AGC GCA GTC GCT TAC CTT C 3'
R 5' GCA TAC GAC GAC GAC TAC GA 3'
XOO1664 F 5' CAC GCG TCT ACT GGG AAG AT 3'
R 5' AAC ACG TCA TAC AGC GCA TC 3'
XOO4468 F 5' ACG ATT TCG ACC TGG ACC AC 3'
R 5' ACA AGG ACG CCG AAA AGA T 3'
XOO1458 F 5' CCA GCG TTC CAT CAC TAC G 3'
R 5' AGG GTA ATT AAC CGG CTT CG 3'
XOCORF1456 F 5' AAT GAC AAT GAG GGC ATC AA 3'
R 5' ACT GAT TTG CGT TGT CGT TG 3'
XOCORF3144 F 5' GCA GAC GTT CGA CAC TTT CA 3'
R 5' GCC TGT GTC TGC GAC TTG TA 3'
XOCORF3137 F 5' ACG ACC GTA TCC AAC CAG AC 3'
R 5' AAC ATG CTG CGG ATT TCT TC 3'
XOCORF2869 F 5' AGT CGT TCG TAC CAG CCA TC 3'
R 5' GCT CAC CTC CTG CTT GTA GC 3'
XOCORF0857 F 5' GCC AGC TTG AAA GTC AGC TC 3'
R 5' CAT TTG CAG CAT TGG TGA AG 3'
XOCORF0690 F 5' TTC CTT TTC GCC TGG AGT T 3'
R 5' TTC ATC GAC ACC GTC ATT G 3'
XOCORF4060 F 5' AAG TCA GTC CCG GTC AAG GT 3'
R 5' ATT CCT CCA CCA TCT CGT TG 3'
XOCORF0488 F 5' GAC GTT CCG ACC AAT CTG TT 3'
R 5' CTG CCC GAT CTT GAT CAT CT 3'
XOCORF2820 F 5' AAG ACC ACC CAC AAG CTG TT 3'
R 5' GAC CCT TGA TGG TCA GAT CG 3'
XOCORF4434 F 5' AAT CTG GCC AAC GTC GAT AC 3'
R 5' AGC TGG ATC ATT TTC CAC CA 3'
XOCORF0026 F 5' ATG GTG GAA AGC CTC AAC AC 3'
R 5' GCC AGG ATA TTG GTC TGG AA 3'
XOCORF0775 F 5' AAA CTC TCG TGC TTG GTG CT 3'
R 5' CAG CGT ATT CGT AGG TGA CG 3'
XOCORF4022 F 5' CAG CAT TCG CTG AAG GAA CT 3'
R 5' AAA TAC GGC ACC TTG TGC TC 3'
XOCORF3816 F 5' TAT ACT GGT CGC TGC TGG TG 3'
R 5' CGG TAA GTC ACC TCG TAG CC 3'
XOCORF1384 F 5' CCA AGA TCC GCA AGA AGA AG 3'
R 5' GGA TCA GCT TTT CGA TCT GC 3'
XOCORF2448 F 5' GCT CAC TTA ATT CGC GCT TC 3'
R 5' AAC GAG CTG CTT AGC GTT GT 3'
  1. aThe gene ID is according to the primary annotation obtained from the Comprehensive Microbial Resource. XOO designations represent IDs of genes from Xanthomonas oryzae pv. oryzae KACC 10331 and XOCORF designations represent IDs of genes from Xanthomonas oryzae pv. oryzicola BLS256.