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Table 1 Optimization of hybridization temperature and probe amounts

From: A two-genome microarray for the rice pathogens Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola and its use in the discovery of a difference in their regulation of hrp genes

Amount of probea (pmol) 10 20 30 40 50
HighPMTb 0.69c 0.70 0.70 0.92 0.93
LowPMT 0.72 0.73 0.71 0.93 0.94
Hybridization temperature (°C) 42 44 48 52  
HighPMT 0.87 0.73 0.67 0.77  
LowPMT 0.87 0.72 0.73 0.76  
  1. a Probes were generated from RNAs extracted from Xoo cultured in PSB.
  2. b PMT represents power of the scanner photomultiplicator that can influence ratio experimental estimation, dynamical range extension or saturation of highly expressed genes. In this study, we tuned to two (high and low) PMT levels to acquire raw slide pictures.
  3. c value of correlation coefficient in self-self hybridization.