Figure 3
From: Phagocytosis of Aspergillus fumigatus conidia by primary nasal epithelial cells in vitro
Intracellular trafficking of A. fumigatus and P. chrysogenum conidia within HNEC phagosomes after 4 h incubation. After fixation and permeabilization, cells were labeled with specific mouse monoclonal anti-LAMP1 antibody (mAb) and secondary antibody conjugated to Texas-red. The lower figures are the corresponding phase contrast images. (A and B) A. fumigatus conidia; (C and D) P. chrysogenum conidia; (E and F) control HNEC labeled with mouse IgG1 mAb. Arrows indicate conidia stained positive for LAMP1. Bar = 2 μm.