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Figure 4 | BMC Microbiology

Figure 4

From: Antibodies from women urogenitally infected with C. trachomatis predominantly recognized the plasmid protein pgp3 in a conformation-dependent manner

Figure 4

Detection of endogenous chlamydial antigens in C. trachomatis serovar L2-infected cells by fusion protein-purified human IgG (panels a, c, e & g) or antigen-specific mouse antibodies (panels b, d, f & h). The primary antibody staining was visualized with either a goat anti-human IgG Cy3 or goat anti-mouse IgG Cy3 conjugates (red). The chlamydial inclusions were visualized with a rabbit anti-chlamydial organism antibody plus a goat anti-rabbit IgG Cy2 conjugate (green). The DNA was visualized by the Hoechst DNA dye (blue). Note that both the pgp3 and CPAF-purified human IgG localized the corresponding endogenous antigens inside the host cell cytosol of the C. trachomatis-infected cells.

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BMC Microbiology

ISSN: 1471-2180

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