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Table 3 Characterization of class I integrons encoded by S. Heidelberg and E. coli. Integrons were amplified using primers intAF and intAR [25] and sequenced using these same primers, and if necessary to achieve complete coverage, intAF2a, intAF2b, intAF3, intAF4, intAR3 and intAR4 (Table 2).

From: Genetic characterization of clinical and agri-food isolates of multi drug resistant Salmonella enterica serovar Heidelberg from Canada

Isolate

Source

Integron length (kbp)

Location

Genes encodeda

01–7169

Human – stool

1.2

Unknown b

aadA2

02–4660

Human – stool

1.2

HMW plasmid

dhfRA1

02–5785

Human – stool

1.2

Unknown c

nd

03–0845

Human – stool

3.0

HMW plasmid

aadB, cmlA

05–4264

Bovine – passive

1.2

Unknown c

dhfRA1

E. coli 830

Chicken – abattoir

1.0

HMW plasmid

aadA1

E. coli 831

Chicken – abattoir

1.0

HMW plasmid

aadA1

E. coli 833

Chicken – abattoir

1.0

HMW plasmid

aadA1

  1. a aadA1, streptomycin adenyltransferase; aadA2, streptomycin/spectinomycin adenyltransferase; aadB, aminoglycoside adenyltransferase; cmlA, chloramphenicol/florphenicol efflux; dhfrA1, dihydrofolate reductase; nd, not determined
  2. b no HMW plasmids were observed in this strain
  3. c HMW plasmids were observed, but could not be isolated from this strain after transformation attempts