Table 2 Oligonucleotides used in this study
Primer | Targeta | Sequence 5'-3' | Product Size (bp) | Annealing Temp | Reference |
|---|---|---|---|---|---|
aadA1F | aadA1 | TATCAGAGGTAGTTGGCGTCAT | 484 | 54°C | [25] |
aadA1R | Â | GTTCCATAGCGTTAAGGTTTCATT | Â | Â | Â |
aadA2F | aadA2 | TGTTGGTTACTGTGGCCGTA | 712 | 61°C | [25] |
aadA2R | Â | GATCTCGCCTTTCACAAAGC | Â | Â | Â |
cmy-2-1 | bla cmy-2 | ACACTGATTGCGTCTGACG | 1143 | 60°C | [26] |
cmy-2–2 |  | AATATCCTGGGCCTCATCG |  |  |  |
cmy-2–3 |  | AGTTCTGGCCAGTATTTCG | n/a | sequencing |  |
cmy-2–4 |  | TGCAACCATTAAAACTGGC | n/a | sequencing |  |
cmy-2–5 |  | TTCCTTTTAATTACGGAAC | n/a | sequencing |  |
dhfRA1F | dhfRA1 | GTGAAACTATCACTAATGGTAGCT | 470 | 50°C | [27] |
dhfRA1R | Â | ACCCTTTTGCCAGATTTGGTACCT | Â | Â | Â |
fimAF | fimA | TCAGGGAGAAACAGAAAACTAAT | 760 | 55°C | [11] |
fimAR | Â | TCCCCGATAGCCTCTTCC | Â | Â | Â |
fldA-R | fldA | TTAGGCGTTGAGGATGTCG | 530 | 55°C | This study |
fldA-F | Â | GCAATCACTGGCATCTTTTTC | Â | Â | Â |
floRF | floR | AACCCGCCCTCTGGATCAAGTCAA | 548 | 60°C | [25] |
floRR | Â | CAAATCACCGGCCACGCTGTATC | Â | Â | Â |
furR-F | furR | AAAGAAGGCTGGCCTGAAAG | 452 | 50°C | This study |
furR-R | Â | TTATTTAGTCGCGTCATCGTG | Â | Â | Â |
intAF | 3' CS | GGCATCCAAGCAGCAAG | variable | 52°C | [25] |
intAR | 5' CS | AAGCAGACTTGACCTGG | Â | Â | Â |
intAF2a | integron | AACCTTTTTGGCCTCCAG | n/a | sequencing | This study |
intAF2b | Â | CCTCCAGCGCTTGTGTAG | n/a | sequencing | Â |
intAF3 | Â | TCAGTGTTAGTCCCATCTCC | n/a | sequencing | Â |
intAF4 | Â | ACTTTAGTTGGCGGTACTCC | n/a | sequencing | Â |
intAR3 | Â | CCATAAAACGAGCCGTAAAC | n/a | sequencing | Â |
intAR4 | Â | CAAACCTAGAAACGCAAAGA | n/a | sequencing | Â |
manBF | manB | CATAACCCGATGGACTACAACG | 893 | 55°C | [11] |
manBR | Â | ACCAGCAGCCACGGGATCAT | Â | Â | Â |
mdhF | mdh | GATGAAAGTCGCAGTCCTCG | 849 | 50°C | [11] |
mdhR | Â | TATCCAGCATAGCGTCCAGC | Â | Â | Â |
miaB-R | miaB | TAGAATCCTACGCCCAGCTC | 1424 | 55°C | This study |
miaB-F | Â | GGGCTGTCAGATGAACGAG | Â | Â | Â |
pefA | pefA | TCACTGTCTCCTGGGCTTCT | 219 | 50°C | This study |
pefA | Â | CTTCAGTCTGGCCACCTTTC | Â | Â | Â |
potE-R | potE | AATTCAAAACGCGGTGAGAC | 1319 | 55°C | This study |
potE-F | Â | TGTCGTGCAGCTCACAATTC | Â | Â | Â |
rep1IF | repI | CGAAAGCCGGACGGCAGAA | 139 | 50°C | [17] |
rep1IR | Â | TCGTCGTTCCGCCAAGTTCGT | Â | Â | Â |
repA/CF | repA | GAGAACCAAAGACAAAGACCTGGA | 465 | 50°C | [17] |
repA/CR | Â | ACGACAAACCTGAATTGCCTCCTT | Â | Â | Â |
spvC1 | spv | AACTCCTTGCACAACCAAATG | 230 | 50°C | This study |
spvC1 | Â | ACCATATCCCTGAGCACACTG | Â | Â | Â |
strAF | strA | AGCAGAGCGCGCCTTCGCTC | 684 | 61°C | [25] |
strAR | Â | CCAAAGCCCACTTCACCGAC | Â | Â | Â |
sul1F | sul1 | TCACCGAGGACTCCTTCTTC | 631 | 60°C | [25] |
sul1R | Â | AATATCGGGATAGAGCGCAG | Â | Â | Â |
tetAF | tetA | GCTACATCCTGCTTGCCTTC | 210 | 70°C | [28] |
tetAR | Â | CATAGATCGCCGTGAAGAGG | Â | Â | Â |
tetBF | tetB | TTGGTTAGGGGCAAGTTTTG | 659 | 64°C | [28] |
tetBR | Â | TTGGTTAGGGGCAAGTTTTG | Â | Â | Â |
tetGF | tetG | CCGGTCTTATGGGTGCTCTA | 693 | 56°C | [25] |
tetGR | Â | CCAGAAGAACGAAGCCAGTC | Â | Â | Â |
thdFF | thdF | TTGATTTTCCGGATGAGGAG | 1553 | 55°C | This study |
thdFR | Â | AGGCATTGACAAAGGTCAGG | Â | Â | Â |