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Table 3 Proteins identified in the OMV fraction of cultures grown in XVM2 medium.

From: Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris

Section Protein Acc.No. MW(cal) Size range Coverage (ESI-MS/MS) Score (ESI-MS/MS) Coverage (MALDI) Score (MALDI)
1 TonB-dependent receptor with signalP AAM 42139 100858 80–150 8 311 10 134
  XadA1 Xanthomonas adhesion XadA AAM39974 220132   7 35 - -
  HrpA ATP-dependent RNA helicase AAM42217 151602   6 32 8 92
  RhsD (RhsD protein) with signalP AAM39448 164320   5 32 7 66
2 FpvA TonB-dependent outer membrane ferripyoverdine receptor with signalP AAM42628 81083 60–100 9 162 12 118
  TonB-dependent outer membrane receptor AAM41663 91909   4 61 8 86
  HrpF (HrpF protein) AAM40515 98836   6 35 14 84
  AvrBs2 (avirulence protein) AAM39371 78471   10 33 - -
  FasD outer membrane usher protein AAM40677 86678   6 30 - -
3 OmpA family outer membrane protein with signalP AAM40245 39338 40–60 28 505 21 97
  XynB exported xylan 1,4-beta-xylosidase with signalP AAM43196 58311   5 34 - -
  UptE Outer membrane protein with signalP AAM39911 31591   15 33 19 56
  AvrBs1 avirulence protein AvrBs1 AAM41388 49789   8 31 13 68
4 OmpA family outer membrane protein with signalP AAM40245 39338 30–40 18 113 20 102
  Egl exported cellulase with signalP AAM42791 52209   4 39 9 86
  HrcU HrcU protein AAM40528 38694   8 30 12 57
  HrpX AraC-type transcriptional regulator HrpX AAM40465 52250   6 30 18 83
  HrcN HrcN protein AAM40534 47759   12 29 10 58
5 OmpW3 OmpW family outer membrane protein AAM42791 23494 25–30 7 55 7 67
  HrpB4 HrpB4 protein AAM40532 22919   4 36 8 59
6 OmpW3 OmpW family outer membrane protein AAM42791 23494 20–25 7 48 7 71
  XpsH General secretion pathway protein AAM39979 18208   20 47 17 61
7 Putative exported protein with signalP AAM40594 10647 10–20 15 245 21 117
  UptD outer membrane protein with signalP AAM39910 17116   12 27 17 74
  1. The section indicates the gel-section excised from the corresponding SDS-PAGE. The values indicated under MW(cal) gives the calculated molecular weight of the identified proteins. The size range relates the excised gel-section with regard to the molecular weight standard on the SDS-PAGE. Score (MOWSE-score) and coverage (sequence coverage) relate to the two approaches (ESI-MS/MS or MALDI-TOF-MS) utilized for the identification of the proteins on the SDS-PAGE.