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Table 2 Results of real-time versus conventional PCR assay. A total of 247 follow up samples of animal origin were analyzed by conventional as well as real-time PCR. Numbers of positive and negative results for each PCR assay are shown.

From: High throughput detection of Coxiella burnetii by real-time PCR with internal control system and automated DNA preparation

  Real-time PCR positive Real-time PCR negative total
PCR positive n = 38 n = 5 n = 43
PCR negative n = 32 n = 172 n = 204
total n = 70 n = 177