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Figure 1 | BMC Microbiology

Figure 1

From: Lsa21, a novel leptospiral protein binding adhesive matrix molecules and present during human infection

Figure 1

Analysis of Lsa21 recombinant protein from NaCl-induced E. coli Bl21-SI by SDS-PAGE and Circular dichroism spectrum. In A. SDS-15% PAGE showing lane 1 – molecular weight protein marker (kDa), lane 2 – non-induced culture, lane 3 – induced culture, lanes 4 and 5 – supernatant and inclusion body pellet after bacterial cell lysis and centrifugation, respectively, lane 6 – purified protein eluted from Ni+2 – charged chelating sepharose column with 1 M imidazole. In B. CD spectrum of Lsa21 protein depicting both α-helical and β-sheets in its secondary structure composition. Far-UV CD spectrum is presented as an average of five scans recorded from 190 to 260 nm.

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