Table 3 Oligonucleotide primers used in this study.
Primer | Sequence (5'-3')a | Application |
|---|---|---|
CM-030 | GGGAGATCT AGGTCCACATTGGATTGCAGG | pvdS promoter |
CM-038 | CCGCTCGAG GGTGAAGCGCCGTGAATAACCACA | pvdS promoter |
CM-036 | GGAAGATCT CCTGTGGATACTTGTTCCGTCA | pvdL promoter |
CM-037 | CCGCTCGAG ACCAAAGAACGCCGCGACGTAATC | pvdL promoter |
CM-050 | GGAAGATCT CTGCTGGGCGTTGAGGGTCTGG | fpvI promoter |
CM-051 | CCGCTCGAG CGGGAAATGCACAGGCGTTCGGCA | fpvI promoter |
CM-052 | GGAAGATCT CGTCTGCAGCGTCTGGTCCTGGAG | fpvR promoter |
CM-053 | CCGCTCGAG GTCCTTGTAGTTGCTGTAGACCAG | fpvR promoter |
CM-116 | GGGAGATCT GAAGTAGGAACGTGTCACTAGTGG | fpvA promoter |
CM-117 | GGGCTCGAG GTCGAAGTGCCTGCCAGCAAG | fpvA promoter |
CM-110 | CACC GTGTAGGAGGCCTCAGCAAGG | pvdS ORF |
CM-111 | ACATAAGGCGCACATTTCATCTGC | pvdS ORF |
CM-112 | CACC ACTCAGGGACCTAATGCCGC | fpvI ORF |
CM-113 | CATGTGTTGATAGGAAACGAGGAC | fpvI ORF |
CM-114 | CACC CGTGAAGTTAGAATGTGGG | fpvR ORF |
CM-115 | TGGGCGGTTCAGGGCATG | fpvR ORF |
pvdL1 | GAAGATCT TCGAACTTCCCCAAACGCT | pvdL deletion |
pvdL2 | CAGCATGCGGATCCGTTGACGGA ACCCGCAGCGCGGGGATGCC | pvdL deletion |
pvdL3 | TCCGTCAACGGATCCGCATGCTG TGGCGCACCAATGGCTGCT | pvdL deletion |
pvdL4 | GAAGATCT CCAACTCCGCCATCAAATCA | pvdL deletion |