Figure 5
From: OppA, the ecto-ATPase of Mycoplasma hominisinduces ATP release and cell death in HeLa cells
Flow cytometric analysis. Flow cytometric analysis of (i) uninfected HeLa cells (K-), (ii) HeLa cells after UV irradiation (K+), (iii) incubation with M. hominis cells (Mhom), (iv) mycoplasmal membrane protein fraction (mem+), (v) membrane proteins without OppA (mem-); (vi) purified OppA protein (OppA) or (iv) OppAΔP-loop-mutant. HeLa cells (1 × 106/ml) were incubated at 37°C for 2h with 1 μg/ml OppA, then stained with Annexin-V-FITC and 7-AAD and subjected to flow cytometric analysis. Necrotic cells were Annexin-V-FITC (+) and 7AAD (+), whereas the apoptotic cells (marked by circle ) were Annexin-V-FITC (+) and 7AAD (-).