Figure 2
From: OppA, the ecto-ATPase of Mycoplasma hominisinduces ATP release and cell death in HeLa cells
Protein preparations used in the infection assay. A: The different mycoplasma samples; M. hominis (Mhom), membrane proteins (mem+), membrane proteins without OppA (mem-), purified OppA and OppAΔP-loop-mutant; corresponding to 0,5 μg/ml OppA, were separated by SDS-PAGE and subsequently stained with Coomassie Blue. B: Westernblot analysis of the protein samples immunostained with the OppA specific monoclonal antibody BG11.