Recovery of a Burkholderia thailandensis-like isolate from an Australian water source

BMC Microbiology

Table 1 DNA-DNA hybridization using labeled DNA from MSMB 43 and from the Burkholderia thailandensis type strain.

Source of unlabeled DNA	Results with labeled DNA from B. thailandensis ATCC 700388^T		Results with labeled DNA from strain MSMB 43
	RBR^a @65°C	D^b	RBR^a @65°C	D^b
B. thailandensis ATCC 700388^T	100	0.0	91	0.4
MSMB 43	91	4.0	100	0.0

^aRelative Binding Ratio: the amount of double-stranded DNA formed between labeled and unlabeled DNAs from different strains divided by the amount of double-stranded DNA formed between labeled and unlabeled DNA from the same strain. RBR is expressed as a percentage.
^bDivergence within related sequences calculated on the assumption that each 1°C decrease in the thermal stability of a DNA duplex is caused by 1% of unpaired bases within that duplex. D was calculated to the nearest 0.5%.

ISSN: 1471-2180