Figure 4
From: Novel aromatic ring-hydroxylating dioxygenase genes from coastal marine sediments of Patagonia
Phylogenetic analysis of PhnAc- and NahAc-like sequences recovered from the libraries and their closest relatives from the database. (A) PhnAc-type (A. faecalis AFK2) clones and related sequences and (B) NahAc-type clones and related database sequences. Sequences identified in this study are depicted in bold, and named as: the primer set used (Ac) followed by the sample and year from which they originated and their number in the clone library. Clones with = 99% identity in their deduced amino acid sequences were grouped, and the numbers in parentheses refer to the number of clones represented by this sequence. Reference sequences from GenBank include the accession number in brackets. The asterisks show marine isolates. Outgroups used were PhnAc from Burkholderia sp. RP007 [AAD09872] for PhnAc-type (A. faecalis AFK2) sequences (A) and PhnAc from A. faecalis AFK2 [BAA76323] for NahAc-type sequences (B). Bootstrap values were calculated as percentage of 1000 replicates, and only values greater than 50% are shown. The scale bar represents 0.05 inferred amino acid changes per position.