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Table 6 Results obtained for venous leg ulcer sample (V) using shotgun Sanger sequencing (FRACS).

From: Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

Genus species Number seq % Gram Aerotolerance Shape
Pseudomonas sp*. 45 23.20 - aerobic rod
Pseudomonas aeruginosa.* 30 15.46 - aerobic rod
Pseudomonas fluorescens* 20 10.31 - aerobic rod
Enterobacter spp. 19 9.79 - facultative rod
Stenotrophomonas maltophilia. 14 7.22 - aerobic rod
Enterobacter cloacae 10 5.15 - facultative rod
Staphylococcus aureus* 7 3.61 + Facultative anaerobe cocci
Proteus vulgaris 6 3.09 - Facultative anaerobe rod
Shewanella algae 6 3.09 - Facultative anaerobe rod
Proteus mirabilis. 6 3.09 - Facultative anaerobe rod
UWB 4 2.06 unk unk unk
Clostridium perfringens 4 2.06 + anaerobic rod
Klebsiella spp.* 4 2.06 - facultative rod
Serratia marcescens * 3 1.55 - aerobic rod
Clostridium spp. 2 1.03 + anaerobic rod
Helcococcus kunzii 2 1.03 + facultative anaerobe cocci
Staphylococcus spp.* 2 1.03 + Facultative anaerobe cocci
Enterobacter aerogenes 2 1.03 - Facultative anaerobe rod
Bacillus fusiformis. 1 0.52 + aerobic rod
Enterococcus avium* 1 0.52 + Facultative anaerobe cocci
Enterococcus faecalis* 1 0.52 + Facultative anaerobe cocci
Peptococcus spp. 1 0.52 + anaerobic cocci
Achromobacter xylosoxidans 1 0.52 - aerobic rod
Salmonella spp. 1 0.52 - Facultative anaerobe rod
Escherichia coli 1 0.52 - Facultative anaerobe rod
Shigella spp. 1 0.52 - Facultative anaerobe rod
  1. This table provides the identified genera of the bacteria found using shotgun Sanger sequencing (FRACS) in the V sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. The asterisk * identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the V pool. Bacteria that were cultured and were not found using FRACSS include Acinetobacter, Citrobacter, and Corynebacterium. The % represents the percentage of the total sequences analyzed within the sample.