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Table 6 Results obtained for venous leg ulcer sample (V) using shotgun Sanger sequencing (FRACS).

From: Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

Genus species

Number seq

%

Gram

Aerotolerance

Shape

Pseudomonas sp*.

45

23.20

-

aerobic

rod

Pseudomonas aeruginosa.*

30

15.46

-

aerobic

rod

Pseudomonas fluorescens*

20

10.31

-

aerobic

rod

Enterobacter spp.

19

9.79

-

facultative

rod

Stenotrophomonas maltophilia.

14

7.22

-

aerobic

rod

Enterobacter cloacae

10

5.15

-

facultative

rod

Staphylococcus aureus*

7

3.61

+

Facultative anaerobe

cocci

Proteus vulgaris

6

3.09

-

Facultative anaerobe

rod

Shewanella algae

6

3.09

-

Facultative anaerobe

rod

Proteus mirabilis.

6

3.09

-

Facultative anaerobe

rod

UWB

4

2.06

unk

unk

unk

Clostridium perfringens

4

2.06

+

anaerobic

rod

Klebsiella spp.*

4

2.06

-

facultative

rod

Serratia marcescens *

3

1.55

-

aerobic

rod

Clostridium spp.

2

1.03

+

anaerobic

rod

Helcococcus kunzii

2

1.03

+

facultative anaerobe

cocci

Staphylococcus spp.*

2

1.03

+

Facultative anaerobe

cocci

Enterobacter aerogenes

2

1.03

-

Facultative anaerobe

rod

Bacillus fusiformis.

1

0.52

+

aerobic

rod

Enterococcus avium*

1

0.52

+

Facultative anaerobe

cocci

Enterococcus faecalis*

1

0.52

+

Facultative anaerobe

cocci

Peptococcus spp.

1

0.52

+

anaerobic

cocci

Achromobacter xylosoxidans

1

0.52

-

aerobic

rod

Salmonella spp.

1

0.52

-

Facultative anaerobe

rod

Escherichia coli

1

0.52

-

Facultative anaerobe

rod

Shigella spp.

1

0.52

-

Facultative anaerobe

rod

  1. This table provides the identified genera of the bacteria found using shotgun Sanger sequencing (FRACS) in the V sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. The asterisk * identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the V pool. Bacteria that were cultured and were not found using FRACSS include Acinetobacter, Citrobacter, and Corynebacterium. The % represents the percentage of the total sequences analyzed within the sample.
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BMC Microbiology

ISSN: 1471-2180

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