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Table 3 Results obtained for diabetic foot ulcer sample using pyrosequencing (PRAPS).

From: Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

Genus Seq % Gram Aerotolerance Shape
Staphylococcus spp. 10874 29.72 + Facultative anaerobe Cocci
Peptoniphilus spp. 2555 6.98 + Anaerobic Cocci
RhodoPseudomonas spp. 2541 6.94 - Facultative anaerobe Rod
Enterococcus spp. 2341 6.40 + Facultative anaerobe Cocci
Veillonella spp. 1978 5.41 - Anaerobic Cocci
Clostridium spp. 1975 5.40 + Anaerobic Rod
Finegoldia magna 1953 5.34 + Anaerobic Cocci
Haemophilus spp. 1701 4.65 - Facultative anaerobe Rod
Acinetobacter spp. 1301 3.56 - Aerobic Rod
Morganella spp. 1240 3.39 - Facultative anaerobe Rod
Serratia spp. 1125 3.07 - Facultative anaerobe Rod
Proteus spp. 1072 2.93 - Facultative anaerobe Rod
Dialister spp. 1029 2.81 - Anaerobic Rod
Streptococcus spp. 751 2.05 + Facultative anaerobe Cocci
Stenotrophomonas spp. 669 1.83 + Aerobe Rod
Peptococcus niger 588 1.61 + Anaerobic Cocci
UWB 342 0.93 unk Unk Unk
Klebsiella spp. 326 0.89 - Facultative anaerobe Rod
Actinomyces spp. 307 0.84 + Facultative anaerobe Rod
Gordonia spp. 302 0.83 + Aerobic Rod
Delftia spp. 251 0.69 - Aerobic Rod
Gemella spp. 168 0.46 + Anaerobic Cocci
Corynebacterium spp. 157 0.43 + Facultative anaerobe Rod
UWB 143 0.39 unk Unk Unk
UWB 107 0.29 unk Unk Unk
Salmonella enterica 102 0.28 - Facultative anaerobe Rod
Fusobacterium spp. 99 0.27 - Anaerobic Rod
Varibaculum cambriense 54 0.15 + Anaerobic Rod
Enterobacter spp. 52 0.14 - Facultative anaerobe Rod
Bacillus spp. 51 0.14 + aerobic Rod
Eikenella spp. 42 0.11 - facultative anaerobe Rod
Anaerococcus spp. 42 0.11 + anaerobic Cocci
Hydrogenophaga spp. 40 0.11 - aerobic Rod
Alcaligenes faecalis 36 0.10 - aerobic Rod
E coli 32 0.09 - facultative anaerobe Rod
Sphingomonas spp. 26 0.07 - aerobic Rod
Acidovorax spp. 26 0.07 - aerobic Rod
Prevotella spp. 22 0.06 - anaerobic Rod
UWB 20 0.05 unk unk Unk
Eubacterium spp. 20 0.05 + anaerobic Rod
Bacteroides spp. 20 0.05 - anaerobic Rod
UWB 17 0.05 unk unk Unk
UWB 16 0.04 unk unk Unk
Selenomonadaceae spp. 16 0.04 - anaerobic Rod
Brevibacterium spp. 14 0.04 + aerobic Rod
Riemerella spp. 13 0.04 - aerobic Rod
UWB 11 0.03 unk unk Unk
Bradyrhizobium spp. 11 0.03 - aerobic Rod
Pantoea agglomerans 10 0.03 - facultative anaerobe Rod
  1. This table provides the identified genus of the bacteria found using pyrosequencing (PRAPS) in the D sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. UWB refers to unknown wound bacteria or sequences without significant similarity to database sequences. * The asterisk identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the diabetic foot ulcer pool (D sample). Bacteria that were cultured and were not found using PRAPS include Citrobacter and Pseudomonas. % represents the percentage of the total sequences analyzed within the sample.