Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

BMC Microbiology

Table 1 Overview of the phenotypes of microbial populations as determined using pyrosequencing (PRAPS).

	Pressure Ulcer seq	Pressure Ulcer genus	Diabetic Foot Ulcer seq	Diabetic Foot Ulcer genus	Venous Let Ulcer seq	Venous Leg Ulcer genus
Anaerobe	17105	12	10519	15	523	6
Aerobe	3241	9	2740	12	4402	9
Facultative anaerobes	6686	15	22673	19	25226	16
Gram positive	18751	18	22821	20	984	12
Gram negative	8281	17	13111	26	29167	19
Rod	8384	25	14682	34	29729	25
Cocci	18648	12	21250	12	422	6
UWB	440	8	656	7	1724	3

The data from pyrosequencing (PRAPS) is broken down into the number of sequences (seq) from each sample associated with the indicated phenotypic characteristic (anaerobe, gram positive, etc). Also shown are the number of genera that were associated with each of the phenotypic categories. UWB refers to unknown wound bacteria or sequences without significant similarity to a database sequences.

ISSN: 1471-2180