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Figure 6 | BMC Microbiology

Figure 6

From: EndoS from Streptococcus pyogenes is hydrolyzed by the cysteine proteinase SpeB and requires glutamic acid 235 and tryptophans for IgG glycan-hydrolyzing activity

Figure 6

SpeB-hydrolyzed rEndoS loses activity on human IgG. Panel A, rEndoS was incubated with SpeB as described in material and methods at indicated time points and 10 μl of samples were applied on polyacrylamide gel and stained with Coomassie Blue. Panel B, 2 μl sample withdrawn from the same incubations, was incubated with 2 g IgG for 2 hours and IgG hydrolysis was analyzed by SDS-PAGE and LCA-blot.

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BMC Microbiology

ISSN: 1471-2180

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