Table 1 Strains and plasmids used in this study
From: LcrG secretion is not required for blocking of Yops secretion in Yersinia pestis
Strain | Relevant properties | Source or reference |
|---|---|---|
E. coli Novablue Y. pestis | recA1 endA1 hsdR17(rK mK+) suE44 thi-1 gyrA96 relA1 lac [F'proA+ B+ lacIqZΔM15::Tn10] | Novagen |
KIM8-3002 | pCD1 (Lcr+) pMT1 Pla- Smr | [26] |
KIM8-3002.7 | KIM8-3002 ΔlcrG3 [LcrGΔ6-86]a | [19] |
Plasmids | ||
pJM174 | GAL4(768–881) AD LEU2 LcrG Apr | [18] |
pBAD18 | araBAD p cloning vector, Apr | [38] |
pLR001 | LcrG-GAL4(768–881) AD Apr | This study |
pLR002 | pBAD18+lcrG [LcrGΔ2–6]a (M Δ2–6 D E) | This study |
pLR003 | pBAD18+lcrG (polyserine aa 2–6)b (M S S S S S D E) | This study |
pLR004 | pBAD18+lcrG (polyisoleucine aa 2–6)b (M I I I I I D E) | This study |
pLR005 | pBAD18+lcrG (polyisoleucine/serine aa 2–6) b (M S I S I S D E) | This study |
pAraG18 | pBAD18+lcrG (wild type) (M K S S H F D E) | [26] |