Figure 4

Phosphorylation assays with L. biflexa Hklep and Rrlep proteins. (A) For autophosphorylation assays, Hklep was incubated with [γ-³²P]ATP for 1 h 30. The arrow indicates the putative dimer form of Hklep. (B) For phosphotranfer assays, an equal amount of Rrlep protein was added to the phosphorylated Hklep and the reaction was further incubated for 5, 15, 30, and 60 min. Proteins were separated on 12 % SDS-polyacrylamide gel and visualized using autoradiography. The molecular mass is indicated in kDa.