Response of the cytoplasmic and membrane proteome of Corynebacterium glutamicum ATCC 13032 to pH changes

Barriuso-Iglesias, Mónica; Schluesener, Daniela; Barreiro, Carlos; Poetsch, Ansgar; Martín, Juan F

doi:10.1186/1471-2180-8-225

BMC Microbiology

Table 2 Proteins identified in the membrane fractions of C. glutamicum ATCC 13032, with their regulation factors, at different pH conditions

From: Response of the cytoplasmic and membrane proteome of Corynebacterium glutamicum ATCC 13032 to pH changes

								Regulation factors^e
Spot	Name	Accesion no.	Function	L^a	Calculated MW (kDa)	Calculated pI	pH induction^b	6.0/7.0	S.D.^f	9.0/7.0	S.D.^f	9.0/6.0	S.D.^f
1	Sdh CD	cg0445	Succinate dehydrogenase CD	5	28.2	9.82	pH 9.0	0.68^g	0.15	1.30^g	0.06	↑2.04 ^g	0.52
2	Hypothetical protein (7 TMHs)	cg0896	---	7	10.9	4.35	pH 6.0	↑2.02 ^g	0.76	1.18	0.16	0.64^g	0.14
3	Sdh A	cg0446	Succinate dehydrogenase A	a	7.40	5.40	pH 9.0	↓0.48 ^g	0.12	1.26	0.20	↑2.85 ^g	1.07
4	Sdh B	cg0447	Succinate dehydrogenase B	a	2.65	5.43	pH 9.0	0.61	0.18	1.41^g	0.22	↑2.52 ^g	0.77
5	AtpF	cg1364	F0F1-type ATP synthase b subunit	a	20.9	4.97	pH 9.0	↓0.41 ^g	0.16	0.86	0.26	↑2.17 ^g	0.24
6	AtpH	cg1365	F0F1-type ATP synthase delta subunit	a	28.7	5.36	pH 9.0	0.55	0.25	0.85	0.21	↑1.75^g	0.46
7	AtpA	cg1366	F0F1-type ATP synthase alpha chain	a	58.6	4.77	pH 9.0	0.60^g	0.08	0.88	0.11	↑1.47^g	0.16
8	AtpD	cg1368	F0F1-type ATP synthase beta chain^h	a	52.4	4.63	---^c	0.61^g	0.10	0.68^g	0.06	↑ 1.12	0.08
9	GluB	cg2137	Glutamate secreted binding protein	a	31.5	3.89	---^c	0.84	0.36	0.98	0.22	1.30	0.38
10	ClpC	cg2963	ATPase with chaperone activity (heat shock protein)	a	101.5	4.93	pH 6.0	↑ 2.91	2.38	1.00	0.20	0.54	0.24
11	CysK	cg2833	O-Acetylserine (Thiol)-Lyase	c	32.6	4.68	pH 6.0/pH 9.0	↑1.81^g	0.88	↑1.56^g	0.22	0.91	0.42
12	ButA	cg2958	L-2.3-butanediol dehydrogenase/acetoin reductase	c	27.0	4.51	pH 6.0	↑1.58^g	0.37	0.78	0.16	0.59	0.25
13	Putative secreted/membrane protein	cg0752	Uncharacterized BCR	s	49.6	5.06	pH 9.0	↓0.30 ^g	0.03	0.83	0.09	↑2.79 ^g	0.59
14	Pks	cg3178	Polyketide synthase	a	172.2	4.40	---^d	0.90	0.29	0.63^g	0.11	0.77	0.25
15	NarG	cg1344	Nitrate reductase 2 alpha subunit	a	139.2	5.74	pH 9.0	0.58	0.35	0.87	0.22	↑2.63 ^g	1.99
16	Conserved hypothetical protein	cg1556	---	s	33.2	9.70	pH 6.0	↑1.62^g	0.85	1.05	0.14	0.95	0.48
17	SucB	cg2421	Dihydrolipoamide acyltransferase	c	70.7	4.14	pH 6.0	↑3.21 ^g	1.75	1.38^g	0.09	0.58	0.31

^a)Localization; c: cytoplasmic, a: membrane-associated, s: secreted, digits indicate numbers of transmembrane helices.
^b)See discussion.
^c)The statistical results of three biological culture replicates were not significant enough to define an up-regulation at pH 9.0.
^d)The statistical results of three biological culture replicates were not significant enough to define a regulation at pH 6.0 or 9.0.
^e)Regulation factors were obtained from three biological culture replicates for each pH condition. Arrows indicate up- or down-regulation. In boldface are shown the proteins whose ratios change more than two-fold.
^f)Standard deviation; n = 3.
^g)Significant difference (t-test, p < 0.1).
^h)Despite the p-value > 0.1 (0.17), and the 9.0/6.0 ratio is below 2, this protein was regarded as pH regulated by Western-blotting (see Results).

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