Figure 2

Comparative expanded (ampholite range 4.5 to 5.5) 2D-gel analysis of Corynebacterium glutamicum ATCC 13032 cytoplasmic proteins at pH 7.0, control condition (upper panel), versus pH 6.0 and pH 9.0, acid and basic conditions (left and right lower panels, respectively). To increase the resolution of the spots, a linear pH range of 4.5 to 5.5 was applied in the first dimension. Protein spots 4, 5 and 6 identified by MALDI-TOF PMF are shown in more detail in the space between upper and lower panels. Molecular masses in kDa are indicated on the right side of the upper panel.