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Table 1 Reproducibility of F-BOX-PCR analysis with HEX and 6-FAM dyes calculated as values of Jaccard's coefficient, in comparison with standard BOX-PCR with separation in agarose gel.

From: Fluorescent-BOX-PCR for resolving bacterial genetic diversity, endemism and biogeography

Strain Agarose gel HEX-F-BOX 6-FAM-F-BOX
  DNA extraction PCR Gel Overall DNA extraction PCR Injection Overall DNA extraction PCR Injection Overall
B. cereus BC360 0.822 0.554 0.651 0.300 0.985 0.985 1.000 0.971 1.000 1.000 1.000 1.000
CI1-23 n.d.a 0.906 0.906 0.812 n.d. n.d. n.d. n.d. n.d. 0.972 0.972 0.972
CI2-13 0.864 0.952 0.952 0.818 n.d. 0.838 0.983 0.823 0.970 0.994 0.994 0.951
CI2-17 n.d. 0.624 0.811 0.500 n.d. 0.944 0.981 0.926 n.d. 0.961 0.980 0.942
CI2-23 0.907 0.905 0.864 0.727 0.961 0.983 0.994 0.933 0.969 0.969 1.000 0.939
CO2-33 n.d. 0.830 0.826 0.750 n.d. n.d. n.d. n.d. n.d. 0.978 1.000 0.978
DS3 0.791 0.597 0.803 0.375 0.971 0.971 0.990 0.923 0.919 0.979 0.992 0.950
E. coli DSM50902 0.927 0.874 0.879 0.737 0.979 0.964 0.993 0.943 0.919 0.977 0.992 0.882
Average 0.862 0.780 0.837 0.627 0.974 0.948 0.990 0.920 0.955 0.979 0.991 0.952
Std. Deviation 0.057 0.161 0.090 0.205 0.010 0.056 0.007 0.051 0.035 0.013 0.010 0.035
  1. a n.d. not determined