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Table 3 Immunoblot results of the Ct recombinant proteins related those of the vector tags.

From: Evaluation of an in silico predicted specific and immunogenic antigen from the OmcB protein for the serodiagnosis of Chlamydia trachomatis infections

Vector   Tag-Ct reactivity/Tag reactivity
PinPoint Xa-1 No. of sera BPT-Ct+/BPT+ BPT-Ct +/BPT- BPT-Ct -/BPT+ BPT-Ct -/BPT-
   12 0 0 6
pGEX-6P-1 No. of sera GST-Ct+/GST+ GST-Ct+/GST- GST-Ct-/GST+ GST-Ct-/GST-
   2 2 0 14
  1. BPT: biotin purification tag expressed using the native PinPoint Xa-1 vector, BPT-Ct: the C-terminal (Ct) recombinant protein expressed using the PinPoint Xa-1 vector, GST: glutathione-S-transferase expressed using the native pGEX-6P-1 vector, GST-Ct: the C-terminal (Ct) recombinant protein expressed using the pGEX-6P-1 vector.