Table 1 Oligonucleotides used in the qRT-PCR reactions
mRNA target | Oligonucleotide (F: forward; R: reverse) | Final concentration (nM) | Amplicon length (bp) | Source |
|---|---|---|---|---|
β-actin | F: 5'-GGATGCAGAAGGAGATCACTG-3' | 300 | 90 | [74] |
| Â | R: 5'-CGATCCACACGGAGTACTTG-3' | 300 | Â | Â |
IL-1β | F: 5'-ACAGATGAAGTGCTCCTTCCA-3' | 300 | 73 | [74] |
| Â | R: 5'-GTCGGAGATTCGTAGCTGGAT-3' | 300 | Â | Â |
IL-6 | F: 5'-ACAGCCACTCACCTCTTCAG-3' | 300 | 120 | This work |
| Â | R: 5'-GTGCCTCTTTGCTGCTTTCAC-3' | 300 | Â | Â |
IL-8 | F: 5'-GAACTGAGAGTGATTGAGAGTGGA-3' | 900 | 134 | [75] |
| Â | R: 5'-CTCTTCAAAAACTTCTCCACAACC-3' | 300 | Â | Â |
MIP-1β | F: 5'-CCAAACCAAAAGAAGCAAGC-3' | 900 | 311 | [76] |
| Â | R: 5'-AGAAACAGTGACAGTGGACC-3' | 300 | Â | Â |
TNF-α | F: 5'-CCCAGGGACCTCTCTCTAATC-3' | 300 | 84 | [74] |
| Â | R: 5'-ATGGGCTACAGGCTTGTCACT-3' | 300 | Â | Â |