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Table 1 Oligonucleotides used in the qRT-PCR reactions

From: Proinflammatory effect in whole blood by free soluble bacterial components released from planktonic and biofilm cells

mRNA target Oligonucleotide (F: forward; R: reverse) Final concentration (nM) Amplicon length (bp) Source
β-actin F: 5'-GGATGCAGAAGGAGATCACTG-3' 300 90 [74]
  R: 5'-CGATCCACACGGAGTACTTG-3' 300   
IL-1β F: 5'-ACAGATGAAGTGCTCCTTCCA-3' 300 73 [74]
  R: 5'-GTCGGAGATTCGTAGCTGGAT-3' 300   
IL-6 F: 5'-ACAGCCACTCACCTCTTCAG-3' 300 120 This work
  R: 5'-GTGCCTCTTTGCTGCTTTCAC-3' 300   
IL-8 F: 5'-GAACTGAGAGTGATTGAGAGTGGA-3' 900 134 [75]
  R: 5'-CTCTTCAAAAACTTCTCCACAACC-3' 300   
MIP-1β F: 5'-CCAAACCAAAAGAAGCAAGC-3' 900 311 [76]
  R: 5'-AGAAACAGTGACAGTGGACC-3' 300   
TNF-α F: 5'-CCCAGGGACCTCTCTCTAATC-3' 300 84 [74]
  R: 5'-ATGGGCTACAGGCTTGTCACT-3' 300