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Table 2 Reciprocal complementation between SSR1_SSR8 and Sna BI-Spe I PFGE.

From: Comparative analysis of Mycobacterium avium subsp. paratuberculosis isolates from cattle, sheep and goats by short sequence repeat and pulsed-field gel electrophoresis typing

PFGE type

subdivided by

SSR types

{1-1}

11

7_4, 7_5, 8_4, 8_5, 9_5, 10_5, 11_5, 12_5, 13_4, 13_5, 14_5

{2-1}

7

7_4, 7_5, 8_4, 10_4, 11_4, 12_5, 14_5

{16–47}

2

9_3, 14_3

{61-47}

2

10_3, 14_3

{54–49}

4

11_5, 12_5, 13_5, 14_5

{69-50}

2

7_3, 14_3

{69-54}

2

13_3, 14_3

SSR type

subdivided by

PFGE types

7_3

3

{56-56}, {57-57}, {69-50}

7_4

19

{1-1}, {2-1}, {2–41}, {15-1}, {52-1}, {60-1}, {2–5}, {2–12}, {2–48}, {2–19}, {2–58}, {51–60}, {55-52}, {58–59}, {59–63}, {63-1}, {64-1}, {68-1}, {70-1}

7_5

2

{1-1}, {2-1}

8_4

4

{1-1}, {1–60}, {2-1}, {2-–46}

8_5

2

{1-1}, {53-1}

11_5

2

{1-1}, {54-49}

12_3

2

{67-51}, {71-64}

12_5

3

{1-1}, {54-49}, {2-1}

13_3

3

{66-62}, {79-55}, {69-54}

13_5

4

{1-1}, {1–10}, {54–49}, {62-1}

14_3

5

{16–47}, {61-47}, {65-61}, {69-50}, {69-54}

14_5

4

{1-1}, {2-1}, {1–53}, {54-49}

  1. Number of SSR1_SSR8 types identified within each Sna BI-Spe I PFGE multiplex profile and vice versa, showing how each technique complements each other in subdividing the most prevalent types.
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BMC Microbiology

ISSN: 1471-2180

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