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Table 1 Plasmids

From: Expression of the pstS gene of Streptomyces lividansis regulated by the carbon source and is partially independent of the PhoP regulator

Plasmid Characteristics Reference
pKC796 Shuttle vector for E. coli/Streptomyces Apramycin resistance. Integrative plasmid in Streptomyces. [29]
pKC796Hyg Shuttle vector for E. coli/Streptomyces Hygromycin resistance. Integrative plasmid in Streptomyces. [6]
pINTUF5 pKC796Hyg derivative containing the original pstS gene from S. lividans. [6]
pINTUF2 pKC796 derivative containing the pstS promoter from S. lividans controlling the expression of the xysA xylanase gene from S. halstedii. [13] This study
pINTUF9 pKC796Hyg derivative. The pstS promoter from S. lividans controls the expression of a fusion gene that contains the region of the xysA xylanase gene that encodes the signal peptide (45 amino acids) and the region that encodes the PstS secreted to the supernatant (from amino acid 42 up to the end). This study
pN702GEM3 Shuttle vector for E. coli/Streptomyces neomycin resistance. Multicopy plasmid [30]
pNUF5 pN702GEM3 derivative. The pstS promoter from S. lividans controls the xysA xylanase gene. [6]
pNUF7 pN702GEM3 derivative. It contains the complete pstS gene from S. lividans. [6]
pNX30 pN702GEM3 derivative. The xysA xylanase ORF does not have any promoter. Used as a negative control. This study
pNUF11 pNUF5 derivative. The 29 bp that include the two PHO boxes of the S. lividans pstS promoter have been deleted (deletion includes from-141 to -113). This study
pNUF13 pNUF5 derivative. The distal 186 bp of the pstS promoter have been deleted (from -329 to -144, both included). This deletion eliminates the 8-times degenerated sequence with the consensus: -ACYCASCCMNSV-. This study