Table 1 Plasmids

pKC796

Shuttle vector for E. coli/Streptomyces Apramycin resistance. Integrative plasmid in Streptomyces.

[29]

pKC796Hyg

Shuttle vector for E. coli/Streptomyces Hygromycin resistance. Integrative plasmid in Streptomyces.

[6]

pINTUF5

pKC796Hyg derivative containing the original pstS gene from S. lividans.

[6]

pINTUF2

pKC796 derivative containing the pstS promoter from S. lividans controlling the expression of the xysA xylanase gene from S. halstedii. [13]

This study

pINTUF9

pKC796Hyg derivative. The pstS promoter from S. lividans controls the expression of a fusion gene that contains the region of the xysA xylanase gene that encodes the signal peptide (45 amino acids) and the region that encodes the PstS secreted to the supernatant (from amino acid 42 up to the end).

This study

pN702GEM3

Shuttle vector for E. coli/Streptomyces neomycin resistance. Multicopy plasmid

[30]

pNUF5

pN702GEM3 derivative. The pstS promoter from S. lividans controls the xysA xylanase gene.

[6]

pNUF7

pN702GEM3 derivative. It contains the complete pstS gene from S. lividans.

[6]

pNX30

pN702GEM3 derivative. The xysA xylanase ORF does not have any promoter. Used as a negative control.

This study

pNUF11

pNUF5 derivative. The 29 bp that include the two PHO boxes of the S. lividans pstS promoter have been deleted (deletion includes from-141 to -113).

This study

pNUF13

pNUF5 derivative. The distal 186 bp of the pstS promoter have been deleted (from -329 to -144, both included). This deletion eliminates the 8-times degenerated sequence with the consensus: -ACYCASCCMNSV-.

This study