Figure 4From: Expression of recombinant Clostridium difficile toxin A and B in Bacillus megateriumCytotoxic effect of recombinant toxins. Mouse colonic epithelial CT26 cells in a 96-well plate were exposed to native (□) or recombinant (Δ) TcdA (A) or exposed to native (□) or recombinant (Δ) TcdB (B) for 72 hr. The MTT assay was performed and cell viability was expressed as the percentage of survival cells without exposure to toxins.Back to article page