Table 2 Bacterial strains, plasmids and primers used in this study
Strain, plasmid or primer | Relevant characteristic(s)/primer sequence | Reference(s) or source |
|---|---|---|
Strains | Â | Â |
S. aureus | Â | Â |
SG511 | Susceptible control strain | RKI Berlin, Germany |
SA137/93A | Clinical hVISA isolate; METr, Northern German epidemic MRSA | [18] |
SA137/93G | Spontaneous mutant of SA137/93A, ΔSCC mec (METs), ΔtcaA | |
SG511ΔvraE | vraE-defective mutant of strain SG511 | [20] |
Plasmids | Â | Â |
pUC19gyrB | pUC19 (Ampr), carrying a 560 bp internal fragment of gyrB; external plasmid standard for qRT-PCR | [38] |
Primers | Â | Â |
a) Oligonucleotide primers used for the synthesis of external qRT-PCR standards | ||
vraE-1 | TCTCATATGACATTTAACCATATCGTTTTC | This study |
vraE-2 | TAACTCGAGAATGGTTTTCTTAATCAATTTGTTTG | This study |
vraS-1 | TTACATATGAACCACTACATTAGAACAAT | This study |
vraS-2 | AATAAGCTTATCGTCATACGAATCCTCCT | This study |
b) Oligonucleotide primers used for qRT-PCR | ||
gyrB-297 | TTAGTGTGGGAAATTGTCGATAAT | [39] |
gyrB-547 | AGTCTTGTGACAATGCGTTTACA | [39] |
vraE-1-RT2 | GTAACTGTATTGTGTTTCGCGGC | This study |
vraE-2-RT2 | TGATGGCATTGTTGCCTGTTACC | This study |
vraS-1-RT | GTTGGTTCGGTACTCGCATA | This study |
vraS-2-RT | CTCGAGCTAGTCTTTGACGTTC | This study |