Figure 4

Function of Rns binding sites in vivo. Rns–dependent and Rns–independent expression of β-galactosidase from reporter constructs integrated into the chromosome of K-12 strain MC4100. Cells were harvested from overnight cultures with an optical absorbance between 0.7 and 0.8 at 580 nm. Mutagenized binding sites are designated with allele numbers at the end of each site's name. n ≥ 3