Table 1 Primers used for multiplex PCR to detect and differentiate Salmonella enterica serogroups and serovars
Target gene1 | Nucleotide sequence | Expected Size (bp) |
|---|---|---|
O-antigen multiplex | Â | Â |
abe1 (B) | F: GGCTTCCGGCTTTATTGG | 561 |
| Â | R: TCTCTTATCTGTTCGCCTGTTG | Â |
wbaD-manC (C1) | F: ATTTGCCCAGTTCGGTTTG | 341 |
| Â | R: CCATAACCGACTTCCATTTCC | Â |
abe2 (C2) | F: CGTCCTATAACCGAGCCAAC | 397 |
| Â | R: CTGCTTTATCCCTCTCACCG | Â |
prt (A/D1) | F: ATGGGAGCGTTTGGGTTC | 624 |
| Â | R: CGCCTCTCCACTACCAACTTC | Â |
wzx – wzy (E1) | F: GATAGCAACGTTCGGAAATTC | 281 |
| Â | R: CCCAATAGCAATAAACCAAGC | Â |
H1-1 multiplex | Â | Â |
fliC (i) | F: AACGAAATCAACAACAACCTGC | 508 |
| Â | R: TAGCCATCTTTACCAGTTCCC | Â |
fliC (g,m) | F: GCAGCAGCACCGGATAAAG | 309 |
| Â | R: CATTAACATCCGTCGCGCTAG | Â |
H1-2 multiplex | Â | Â |
fliC (r) | F: CCTGCTATTACTGGTGATC | 169 |
| Â | R: GTTGAAGGGAAGCCAGCAG | Â |
fliC (z10) | F: GCACTGGCGTTACTCAATCTC | 363 |
| Â | R: GCATCAGCAATACCACTCGC | Â |
H2 multiplex | Â | Â |
fljB (I: 1,2; 1,5; 1,6; 1,7) | F: AGAAAGCGTATGATGTGAAA | 294 |
| Â | R: ATTGTGGTTTTAGTTGCGCC | Â |
fljB (II: e,n,x; e,n,z15) | F: TAACTGGCGATACATTGACTG | 152 |
| Â | R: TAGCACCGAATGATACAGCC | Â |