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Figure 1 | BMC Microbiology

Figure 1

From: Rapid screening of Salmonella entericaserovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles

Figure 1

Multiplex PCR for identifying serogroup-specific, Salmonella O antigen biosynthesis gene(s). Lanes1 and 15: 100 bp MW standard; lane 2, multiplex PCR control for five Salmonella serogroups; lane 3: S. enterica serovar Paratyphi A [A]; lane 4: S. enterica serovar Enteritidis [D1]; lane 5: S. enterica serovar Muenchen [C2]; lane 6: S. enterica serovar Hadar [C2]; lane 7: S. enterica serovar Anatum [E1]; lane 8: S. enterica serovar London [E1]; lane 9: S. enterica serovar Infantis [C1]; lane 10: S. enterica serovar Tennessee [C1]; lane 11: S. enterica serovar Saintpaul [B]; lane 12, S. enterica serovar Typhimurium [B]; lane 13: E. coli K12 LE392, negative control; and lane 14: no DNA control. The sizes of the PCR amplicons are 624 bp for serogroup A/D1, 561 bp for serogroup B, 341 bp for serogroup C1, 397 bp for serogroup C2, and 281 bp for serogroup E1.

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BMC Microbiology

ISSN: 1471-2180

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