Strain or plasmid | Relevant properties* | Source or Reference |
---|---|---|
Strains | Â | Â |
B. breve | Â | Â |
UCC2003 | Wild-type parent strain | UCC culture collection |
UCC2003-bilE- | UCC2003 containing the cloning vector pNZ8048 | This study |
UCC2003-bilE+ | UCC2003 containing the cloning vector pNZ8048-bilE+ | This study |
L. lactis subsp. Cremoris | Â | Â |
NZ9000 | L. lactis subsp. cremoris MG1363 carrying nisRK on the chromosome | 10. |
NZ9000-bilE- | NZ9000 containing the cloning vector pNZ8048 | This study |
NZ9000-bilE+ | NZ9000 containing the cloning vector pNZ8048-bilE+ | This study |
E. coli | Â | Â |
DH10B | Cloning host | Invitrogen |
L. monocytogenes | Â | Â |
EGD-e | Wild-type of serotype 1/2a for which the genome sequence is available. | UCC culture collection |
Plasmid | Â | Â |
pNZ8048 | *Cmr, low copy number plasmid. | 37. |
pNZ8048-bile+ | *Cmr, low copy number plasmid, harbouring bilE under the control of the native listerial promoter. | This study |
Primers | Sequence 5'-3' | Â |
BilEF | CATTCTAGA GTTTGTAAGTTATT | Â |
BilER | CAAATTCTTTGTTGAATTCCTGCAG ATAT | Â |
Bif16SF | CCGGATGCTCCATCACAC | Â |
Bif16SR | ACAAAGTGCCTTGCTCCCT | Â |
Lac16SF | TGGCTCAGGACGAACGCTGGCGGC | Â |
Lac16SF | CCTACTGCTGCCTCCCGTAGGAGT | Â |
RT-PCRF | GCTTGATTCCACTGACAACTGG | Â |
RT-PCRR | CTTGTGGTGTTGCTACTTGGAC | Â |