Enhancing bile tolerance improves survival and persistence of Bifidobacterium and Lactococcus in the murine gastrointestinal tract

Table 1 Bacterial strains, plasmids and primers used in this study

Strain or plasmid	Relevant properties*	Source or Reference
Strains
*B. breve*
UCC2003	Wild-type parent strain	UCC culture collection
UCC2003-bilE^-	UCC2003 containing the cloning vector pNZ8048	This study
UCC2003-bilE⁺	UCC2003 containing the cloning vector pNZ8048-bilE⁺	This study
*L. lactis* subsp. *Cremoris*
NZ9000	L. lactis subsp. cremoris MG1363 carrying nisRK on the chromosome	10.
NZ9000-bilE^-	NZ9000 containing the cloning vector pNZ8048	This study
NZ9000-bilE⁺	NZ9000 containing the cloning vector pNZ8048-bilE⁺	This study
*E. coli*
DH10B	Cloning host	Invitrogen
*L. monocytogenes*
EGD-e	Wild-type of serotype 1/2a for which the genome sequence is available.	UCC culture collection
Plasmid
pNZ8048	*Cm^r, low copy number plasmid.	37.
pNZ8048-bile⁺	Cm^r, low copy number plasmid, harbouring bilE* under the control of the native listerial promoter.	This study
Primers	Sequence 5'-3'
BilEF	CATTCTAGA GTTTGTAAGTTATT
BilER	CAAATTCTTTGTTGAATTCCTGCAG ATAT
Bif16SF	CCGGATGCTCCATCACAC
Bif16SR	ACAAAGTGCCTTGCTCCCT
Lac16SF	TGGCTCAGGACGAACGCTGGCGGC
Lac16SF	CCTACTGCTGCCTCCCGTAGGAGT
RT-PCRF	GCTTGATTCCACTGACAACTGG
RT-PCRR	CTTGTGGTGTTGCTACTTGGAC

ISSN: 1471-2180