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Table 3 Oligonucleotide primers and TaqMan probes used in RT-qPCR analysis of gene expression

From: Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates

Gene

Sequences (5'→3')

Source

 

Primer

Taqman Probe (Fam-Tamra)

 

CpsA

F: CTTTGCAGTACAGCAGTTTGTTG

ACTGACCAATCGTTTAAATG

Ref. [25]

 

R: TCTGCTAAAACAGCGACACTGA

  

gyrB*

F: CCAAACCGACTATTCAGCGTTAT

TGAAATGGACGATCATCAGCTGTGGGA

In this study

 

R: TGTTCGGGATCCATGGTTGT

  
  1. *gyrB was used as endogenous control.
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BMC Microbiology

ISSN: 1471-2180

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