Table 3 Primers used in this work
Primer | Direction | Sequence 5' to 3' | Target |
|---|---|---|---|
CPR3 | F | CARACTGGKACDGCHGARGATT | crtR gene |
CPR4 | F | CAAACTGGTACGGCTGAAGATT | crtR gene |
CPR5 | R | WGGDCCRATCATGAYRACTGG | crtR gene |
CPR6 | R | AGGTCCAATCATGACGACTGG | crtR gene |
CPR7 | R | CRGTACCWGGDCCRATCATGA | crtR gene |
CPR8 | R | CCAGTACCAGGTCCAATCATGA | crtR gene |
CPR9 | F | GGATCCGCGACATCGAAGAGTATGAC | crtR gene |
CPR12 | R | GGATCCCTTCCAAGCGAGGTAGTCTT | crtR gene |
CPR13 | R | AGAAGACTGTGCGATCGTGT | crtR gene |
CPR31 | R | TCAAGCAATTGGTGTTGGTC | crtR gene |
CPR56 | R | TCGTTGTGTTGTTGCTATTCG | crtR gene |
CPR3A | F | TACAACGTCGTCGGTAGACA | crtR gene |
CPR7A | R | TATTCCACACCGTGGTGTTC | crtR gene |
CPR3D | F | GGTCTCACTTCTCCAGAGAA | crtR gene |
CPRfwdC | F | GCACAGGAAGTTGGTTGGAT | crtR gene |
CPR1exC | F | GCCACACTCTCCGATCTTGT | crtR gene |
CPREx1fwdb | F | TCAGGACCCTGTACAGTCAGC | crtR gene |
CPREx1rev | R | TCCCAACAGTCGATCCTTGT | crtR gene |
Pef | F | GATATCGGCTCATCAGCCGAC | EF-1α Promoter |
Gpd | R | ATGAGAGATGACGGAGATG | GPDH Terminator |
HR | R | CTATTCCTTTGCCCTCGGAC | hph gene |
HF | F | ATGAAAAAGCCTGAACTCACC | hph gene |
HygSecR | R | GTATTGACCGATTCCTTGCG | hph gene |
HygSecF | F | TCGCCAACATCTTCTTCTGG | hph gene |
M13R | R | GGAAACAGCTATGACCATG | pBluescript SK- |
M13F | F | TGTAAAACGACGGCCAGT | pBluescript SK- |
ACT3 | F | ACTCCTACGTTGGTGACGAG | Actin gene |
ACT4 | R | TCAAGTCTCGACCGGCCAAG | Actin gene |
AST1 | F | GCCACCTACTTTCTCCATATGT | crtS gene |
AST2 | R | GAGCCATGACGTCCAGAGTA | crtS gene |