Table 1 Primers used for the construction of the ΔflgG and ΔmotAB mutants
Primer | Sequence (5' – 3') a |
|---|---|
KO1flgG | ATTATGCATGCCGATGAACTATACCTCTCGTCCG |
KO2flgG | ATATAGAGCTCGCCAGTAACCGATCGACTGTCACG |
KO3flgG | GACGATTAGGTTACATCGGTTTTATCCTCTGTGTC |
KO4flgG | GGATAAAACCGATGTAACCTAATCGTCAATCAG |
KO5flgG | GCAACTGGCTGCTTTGCGAGC |
KO6flgG | GTCCGTGGGTTAACGACACCG |
KO1motAB | TATGCATGCGTTACAAATGCTTGAAAGTGAAACAC |
KO2motAB | ATATAGAGCTCGTGGCTGATGTTACCAATGATGC |
KO3motAB | CGTTACTTTGTCACCTTGGTCGCACGCGATATCCTTTAGC |
KO4motAB | GCTAAAGGATATCGCGTGCGACCAAGGTGACAAAGTAACG |
KO5motAB | GCAGCTTACTAGGGAATCTCGAGTGG |
KO6motAB | CTGCCCGTTGACGTGGCGATCCCG |