Figure 6

EDTA-dependent inactivation of SodC and SodC-F. Cu, ZnSOD samples at a concentration of 0.04 mg/ml were incubated at 37°C in 20 mM Tris-HCl buffer, 0.1 mM EDTA, pH 6.8 (panel A) or pH 8.0 (panel B). Aliquots were withdrawn at indicated times and assayed for residual Cu, ZnSOD activity by the pyrogallol method: SodC (black square), SodC-F (black triangle). Each data point represents the mean of at least three independent measures.