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Figure 5 | BMC Microbiology

Figure 5

From: Regulatory and structural properties differentiating the chromosomal and the bacteriophage-associated Escherichia coli O157:H7 Cu, Zn Superoxide Dismutases

Figure 5

Proteinase K susceptibility of SodC and SodC-F. Proteins were incubated at 37°C in 20 mM Tris-HCl at pH 6.8 (panel A) or at pH 8.0 (panel B), in the presence of 0.1 mg/ml proteinase K. Aliquots were withdrawn at indicated times and immediately assayed for residual Cu, ZnSOD activity by the pyrogallol method: SodC (black square), SodC-F (black triangle). Each data point represents the mean of at least three independent measures.

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