Figure 6
6(a) – Binding of truncated rSlpA proteins to isolated native cell walls of L. brevis ATCC 8287. Full-length rSlpA (lanes 1–4), rSlpA1–145 (lanes 5–8), rSlpA1–189 (lanes 9–12), rSlpA190–435 (lanes 13–16) and rSlpA167–435 (lanes 17–20) were incubated with (lanes 1–2, 5–6, 9–10, 13–14 and 17–18) or without (lanes 3–4, 7–8, 11–12, 15–16 and 19–20) cell walls (CWF), and the pellets and supernatants (*) recovered by centrifugation were analyzed by SDS-PAGE. Lanes 21–22, CWF incubated alone; *, supernatant. Numbers on the left indicate molecular weights in kilodaltons. 6(b) – Effect of treatment of cell walls with TCA at +4°C (b1) or at +37°C (b2) on the binding of rSlpA. Full length rSlpA was incubated with native CWF (lanes 1–2) or with CWF treated with 5% TCA (lanes 3–4) and the pellets and supernatants (*) were analyzed by SDS-PAGE. Lanes 5–6, full-length rSlpA incubated alone, lanes 7–8, TCA-treated CWF incubated alone; *, supernatant. Numbers on the left indicate molecular weights in kilodaltons.