An efficient method for visualization and growth of fluorescent Xanthomonas oryzae pv. oryzae in planta

BMC Microbiology

Table 1 Bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristics	Source or reference
Escherichia coli
DH10B	F-mcrAΔ(mrr-hsdRMS-mcrBC) φ80/acZΔM15ΔlacX74 deoR recA1 endA1 araΔ139Δ(ara, leu)7687galU galKλrpsL(Sp^r)nupGλtonA	Gibco BRL
Xanthomonas oryzae pv. oryzae
PXO99	Philippine race 6 (PR6) strain, Cp^r	[31]
PXO99_GFP	PXO99 with pPROBE-NTpNm, Km^r, Cp^r	This study
Plasmids
pPROBE-gfp	pBBR1 ori, Km^r, broad-host range expression vector	[20]
pPneo-gfp	neo (promoter of neomycin resistant gene) cloned into pPROBE-gfp	This study
pML122	OriV, OriT, Gm^r, pNm (nptll), broad-host range expression vector	[21]

Cp^r: Cephalexin resistance, Sp^r: Spectinomycin resistance, Km^r: Kanamycin resistance, Gm^r: Gentamycin resistance

ISSN: 1471-2180