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Table 3 LAMP primers used

From: Development of a loop-mediated Isothermal amplification assay for sensitive and rapid detection of Vibrio parahaemolyticus

Primer Sequence Gene location (bp)
Tlh-FIP ATG TTT TTA AAT GAA ACG GAG CTC CGG CAA AAA ACG AAG ATG GT (F1c-F2) 392-368 (F1c), 321–339 (F2)
Tlh-BIP ACG TCG CAA AAC GTT ATC CGG CGA AGA ACG TAA TGT CTG (B1-B2c) 406–425 (B1), 487-467 (B2c)
Tlh-F3 AGC TAC TCG AAA GAT GAT CC (F3) 283–302
Tlh-B3 GGT TGT ATG AGA AGC GAT TG (B3c) 511-492
Tlh-LF ACC AGT AGC CGT CAA TG (LFc) 367-351
Tlh-LB TTA GAT TTG GCG AAC GAG A (LB) 445–463
  1. All primers were designed from the sequence of tlh gene of V. parahaemolyticus M36437, submitted to GenBank by Taniguchi et al., 1986 [6].