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Table 3 PCR primers used for the identification and characterization of attaching and effacing Escherichia coli strains

From: Characterization of attaching and effacing Escherichia coli (AEEC) isolated from pigs and sheep

Target Primer Oligonucleotide sequence (5'-3') Reference
astA East11a CCA TCA ACA CAG TAT ATC CGA 27
  East11b GGT CGC GAG TGA CGG CTT TGT  
bfpA EP1 AAT GGT GCT TGC GCT TGC TGC 5
  EP2 GCC GCT TTA TCC AAC CTG GTA  
eae SK1 CCC GAA TTC GGC ACA AGC ATA AGC 22
  SK2 CCC GGA TCC GTC TCG CCA GTA TTC G  
eae- α SK1-LP2 CCC GAA TTC TTA TTT TAC ACA AGT GGC 25
eae- γ SK1-LP3 CCC GAA TTC TTC TTT TAC ACA AAC CGC 25
eae- β SK1-LP4 CCC GTG ATA CCA GTA CCA ATT ACG GTC 22
eae- ε SK1-LP5 AGC TCA CTC GTA GAT GAC GGC AAG CG 22
eae- ζ SK1-LP6B TAG TTG TAC TCC CCT TAT CCC 25
eae- ι SK1-LP7 TTT ATC CTG CTC CGT TTG CT 25
eae-η SK1-LP8 TAG ATG ACG GTA GAC 25
eae-κ SK1-LP10 GGC ATT GTT ATC TGT TGT CT 25
eae-θ SK1-LP11B GTT GAT AAC TCC TGA TAT TTT A 25
EAF EAF1 CAG GGT AAA AGA AAG ATG ATA A 27
  EAF25 TAT GGG GAC CAT GTA TTA TCA  
fliC FliC up CAA GTC ATT ATT AC(AC) AAC AGC C 5
  FliC down GAC AT(AG) TT(AG)GA(AGC) ACT TC(GC) GT  
stx VT1 ATT GAG CAA AAT AAT TTA TAT GTG 27
  VT2 TGA TGA TGG CAA TTC AGT AT  
tir tir-R TAA AAG TTC AGA TCT TGA CAT 26
tir Y-P tirY474-F CAT ATT TAT GAT GAG GTC GCT C  
tir S tisS478-F TCT GTT CAG AAT ATG GGG AAT A  
  1. ast A: gene encoding EAST1
  2. bfp A: gene encoding bundle-forming pili
  3. eae: gene encoding intimin
  4. fliC: gene encoding flagellum
  5. EAF: EPEC adherence factor
  6. tir: gene encoding translocated intimin receptor
  7. stx: gene encoding Shigatoxin