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Table 1 Oci A-domains binding pockets and peptide profiles

From: Recombination and selectional forces in cyanopeptolin NRPS operons from highly similar, but geographically remote Planktothrix strains

  Binding pockets OciA-A1 OciA-A2 OciB-A3 OciB-A4 OciB-A5 OciB-A6 OciC-A7
  NIVA CYA 116 DLGFTGAVCK DFWNIGMVHK DAQSMGAIIK DVENAGVVTK DAFFLGVTFK DAWTIAGVCK DAFFLGVTFK
  NIES 205 DLGFTGAVCK DFWNIGMVHK DAEGMGAIIK DVENAGVVTK DAFFLGVTFK DAWTIAGVCK DAFFLGVTFK
NIVA CYA 116         
Mass Da Side chain AA 1 AA2 AA3 AA4 AA5 AA6 AA7
1138 HO3-SO-CH2-CH(OMe)-COH HTyr Thr HTyr Ahp Ile Phe(Me) Ile
1124 HO3-SO-CH2-CH(OMe)-COH HTyr Thr HTyr Ahp Val Phe(Me) Ile
1124 HO3-SO-CH2-CH(OMe)-COH HTyr Thr HTyr Ahp Ile Phe(Me) Val
1074 HO3-SO-CH2-CH(OMe)-COH HTyr Thr Ile/Leu Ahp Ile Phe(Me) Ile
1010 HO3-SO-CH2-CH(OMe)-COH Ile/Leu Thr Ile/Leu Ahp Ile Phe(Me) Ile
1088 HO3-SO-CH2-CH(OMe)-COH HTyr Thr X Ahp Ile Phe(Me) Ile
1122 HO3-SO-CH2-CH(OMe)-COH HTyr Thr Y Ahp Ile Phe(Me) Ile
NIES 205         
Mass Da Side chain AA 1 AA2 AA3 AA4 AA5 AA6 AA7
1138* HO3-SO-CH2-CH(OMe)-COH Htyr Thr Htyr Ahp Ile Phe(Me) Ile
1074 HO3-SO-CH2-CH(OMe)-COH Htyr Thr Ile/Leu Ahp Ile Phe(Me) Ile
1128** HO3-SO-CH2-CH(OMe)-COH Htyr Thr HcAla Ahp Ile Phe(Me) Ile
  1. The binding pocket residues of the NIVA CYA 116 and NIES 205 A-domains were identified by comparison to the GrsA-Phe A-domain (Residue 235, 236, 239, 278, 299, 301, 322, 330, 331, 517). The Oci-A3 binding pockets are different (in grey) between the two strains, and the divergent amino acids are shown in bold. The composition of cyanopeptolins produced by NIVA CYA 116 and NIES 205 and their molecular weights (M+H+) are shown with amino acids correlated with the putative binding pockets. HcAla is 3-(4'-hydroxy-2'-cyclohexenyl) alanine, and X and Y is unidentified amino acid derivates. Mutual peptides in NIVA CYA 116 and NIES 205 are highlighted in dark grey and light grey. See [Additional file 1 figure 1, 2 and 3 for the peptide structure and more details on MS data.* peptide named oscillapeptin E and **peptide named oscillapeptin D