Figure 4

Acetone levels associated with mouse gastric tissue. Stomach acetone levels were determined for three mice after sacrificing the animals and immediately removing their stomachs (post-mortem), and for four mice that were anesthetized after which their stomachs were removed (pre-mortem). Excised mouse stomachs were placed immediately in sealed vials that were then placed on ice for at least 30 min to allow acetone associated with the gastric tissue to equilibrate with the gas phase. Acetone levels in the gas phases of the vials were measured by gas chromatography and estimated from standard curves generated for each vial. Each value represents an average of at least three measurements and error bars indicate the standard deviations for each sample.