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Table 5 Primers for amplifying exogenous DNA sequences of selected representative phages

From: Evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (Ig)-binding proteins (IBPs) with four kinds of Ig molecules

Name

Description

Sequence (5' → 3')

5SNco-u

Forward amplifying primer

TATCCATGG*CTGCGGCCCAGCCGGCCTCT

5SNoG-d

Reverse amplifying primer

CCTGCGGCCGCAACTGCCGCCGCC

B-S-U

Forward sequencing prime

GGA TCC GAG CTC AGG CCT GTC GAC GGT ACC GTT

S-H-D

Reverse sequencing primer

GAG CTC AAG CTT ACC AGA TCC ACC ACC GCC GGT ACC

  1. *: Underlined part represents Nco I recognition site.