Strain/Plasmid | Description | Source/Reference |
---|---|---|
Strains | Â | Â |
E2348/69 | Wild-type EPEC strain EPEC (O127:H6) isolated from an infant with gastroenteritis | [27] |
MC4100 | Wild-type E. coli K-12 lab strain F- araD139 Δ(argF-lac)U169 rpsL150(StrR) relA1 flbB5301 deoC1 ptsF25 rbsR | [28] |
TOP10 cells | Commercially available competent cells for cloning F- mcrA Δ(mrr-hsdRMS-mcfBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu) 7697 galU galK rpsL endA1 nupG (StrR) | Invitrogen Canada Inc. |
TAM1 λpir | Commercially available competent cells of E. coli λlysogen that provide all trans acting and mobilization factors required for the replication and mobilization of λPi dependent plasmids mcrA Δ(mrr-hsdRMS-mcfBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu) 7697 galU galK rpsL endA1 nupG λpir | Active Motif |
SM10 λpir | E. coli SM10 λlysogen that provide all trans acting and mobilization factors required for the replication and mobilization of λPi dependent plasmids | [29] |
NH1 | TOP10 (pUC19 ΔhsdR) | This study |
NH3 | SM10 λpir (pCVD442 ΔhsdR) | This study |
NH4 | E2348/69 ΔhsdR | This study |
LP69 | MC4100 nadA::Tn10 | Lab stock |
Plasmids | Â | Â |
pACYC184 | 5 kb low copy number cloning vector | [16] |
pUC19 | Cloning vector | Invitrogen Canada Inc. |
pUC19 ΔhsdR | pUC19 carrying ΔhsdR construct | This study |
pCVD442 | Cloning vector requiring λ Pi protein to replicate, carries sacB for negative selection (AmpR) | [10] |
pCVD442 ΔhsdR | pCVD442 carrying ΔhsdR construct | This study |
pJW15 | Broad host range promoterless lux reporter plasmid | |
pLAFR1 | Low copy number, broad host range plasmid | [17] |
pNLP10 | Low copy number lux reporter plasmid, pSC101 origin |